DAPI staining of Hela cancer cells without treating by L. plantarum 5BL supernatant

Segmentation of HeLa cells in phase-contrast images with and without support of DAPI stained cell nuclei

Microscopy is an essential tool for the observation of microbial effectors in host cells. Manual evaluation of the acquired image data is elaborate and error prone. Thus, in order to increase objectivity and reproducibility of results, an automated evaluation of the experiment is performed. The present study evaluates methods to delineate HeLa cells depicted in phase-contrast (PC) micrographs, ...

Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining "2279

Single-cell cell cycle analysis is an emerging technique that requires detailed exploration of the image analysis process. In this study, we established a microfluidic single-cell cell cycle analysis method that can analyze cells in small numbers and in situ on a microfluidic chip. In addition, factors that influenced the analysis were carefully investigated. U87 or HeLa cells were seeded and a...

Use of DAPI staining for quantitation of cell chemotaxis.

Double staining (CTC-DAPI) for detection and enumeration of viable but non-culturable Campylobacter jejuni cells.

Direct microscopic enumeration of viable Campylobacter jejuni cells (ie, respiring bacteria) were performed in both culturable and non-culturable states. Five different C jejuni strains were used, including a reference strain, ATCC 33291. Cells from all five strains were incubated alone with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), a redox dye. It was reduced by an electron transport cha...

Lymphocytosis Induced in i l ice by Supernatant Fluids of

Intravenous injection into mice of phase I Bordetella pertussis culture supernatants produces a marked lymphocytosis. The evolution of lymphocytosis was correlated with histopathological alterations in the lymphoid organs. The early phase, 1 7 hr to day 3, was associated with massive depletion of both lymphocytes from the white pulp of the spleen and of cortical thymocytes. There was a correspo...